Determination of adriamycin by enzyme immunoassay.
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DETERMINATION OF SERUM CORTISOL LEVEL BY A DIREC T ENZYME IMMUNOASSAY USING PENICILLINASE AS LABEL
Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...
متن کاملDetermination of glycated albumin by enzyme-linked boronate immunoassay (ELBIA).
A new affinity method for quantification of glycated albumin by an enzyme-linked boronate-immunoassay (ELBIA) has been established, based on the interaction between boronic acids and the cis-diols of glycated human serum albumin (HSA) trapped by anti-HSA antibody. To evaluate the ELBIA, we first examined the accuracy of the conventional boronate affinity chromatographic (BAC) method. In the BAC...
متن کاملDirect determination of plasma endothelin-I by chemiluminescence enzyme immunoassay.
A highly sensitive sandwich-type chemiluminescence enzyme immunoassay for plasma endothelin-I (ET-1), involving no extraction steps, has been developed. Two populations of polyclonal antibodies were used in the present study: One is specific to the C-terminus of the endothelin family of peptides; the other, a Fab' fragment against the N-terminal core region of ET-1, is coupled with horseradish ...
متن کاملdetermination of serum cortisol level by a direc t enzyme immunoassay using penicillinase as label
serum cortisol level was measured by an enzyme immunoassay (eia) using the enzyme penicillinase as a label without prior extraction and purification. polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-cmo) conjugated to bovine serum albumin (bsa). this antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticosteron...
متن کاملRadioimmunoassay and automated enzyme immunoassay for the determination of thyroxine.
The EMIT homogeneous enzyme immunoassay for the determination of thyroxine (T4) was adapted to an automatic analyser (Type Kem-O-Mat) and compared with a conventional radioimmunoassay. Sera from 92 patients whose thyrometabolic status was being examined, and control sera with 3 different T4 concentrations were analysed by both methods. Day-to-day analyses of the control sera gave variation coef...
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ژورنال
عنوان ژورنال: Journal of Pharmacobio-Dynamics
سال: 1983
ISSN: 0386-846X,1881-1353
DOI: 10.1248/bpb1978.6.588